Current - Issue

Year 2026 · Volume 6 · Issue 5

Review Article

A Critical Review of UV Spectrophotometry and HPLC Methods for the Quantitative Determination of Metformin Hydrochloride and Glimepiride

Ramakrishnan.M1 Manoj V2 Gokulamanikandan M3 Saravanan. V.S4 Prabhu.S5
1 2 3 4 5 Department of Pharmaceutical Analysis, The Erode College of Pharmacy, Veppampalayam, Erode, Tamilnadu, India.

Published Online: September-October 2026

Pages: 214-220

References

1. Dange YD, Honmane SM, Bhinge SD, Salunkhe VR, Jadge DR. Development and Validation of UV-Spectrophotometric Method for
Estimation of Metformin in Bulk and Tablet Dosage Form. Indian Journal of Pharmaceutical Education & Research. 2017;51(4S):S754-
S760.
2. Mishra K, Soni H, Nayak G, Patel SS, Singhai AK. Metformin Hydrochloride: Method Development and Validation in Tablet Dosage
Form. E-Journal Chemistry. 2011;8(3):1309-1313.
3. Cholke P, Shirsath M, Temak Y, Kagde A, Lagad R, Development and Validation of UV-Vis Spectroscopic Method for Simultaneous
Estimation of Saxagliptin Hydrochloride and Metformin Hydrochloride in Tablet Dosage Form. Journal of Research in Pharmacy and
Pharmaceutical Sciences-International 2018;3(4):31-34.
4. Konari SN, Jacob JT, Prakash V. Stability Indicating UV Spectrophotometric Method For Linagliptin and Metformin in Pharmaceutical
Dosage Form. Pharm Methods. 2017;8(2):121–126.
5. Kharbade S, Asnani A, Pratyush K. Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of
Metformin HCl and Repaglinide in Pharmaceutical Formulation. J. Drug Delivery and Therapeutics 2019;9(3):344-347.
6. Saxena P, Raghuwanshi AS, Jain UK, Patel A, Gupta N. UV spectrophotometric method for the quatitation of metformin hyrochloride in
pharmaceutical dosage form. Oriental J. Chem. 2010;26(4):1553-1556.
7. Ganesh K, Nikitha G, Sireesha D, Vasudha B. Development and Validation of UV Spectrophotometric Method for Simultaneous
Estimation of Metformin and Glipizide in Tablet Dosage Form. International Journal of Applied Pharmaceutical Sciences and Research.
2016; 1(2): 56-59.8. Shankar MB, Modi VD, Shah DA, Bhatt KK, Mehta RS, Geetha M, Patel BJ. Determination of Pioglitazone Hydrochloride and Metformin
Hydrochloride in Tablets by Derivative Spectrophotometry and Liquid Chromatography Techniques. Journal of AOAC Int.
2005;88(4):1167-1172.
9. Goyal PK, Solanki MS. Simultaneous estimation of Teneligliptin and Metformin Hydrochloride in tablet dosage form by UV
spectrophotometric method. Chemistry Research Journal. 2021;6(6):160-165.
10. Bhargavi S, Suryasagar G, Sowmya DK, ashok K, Nama S. UV Spectrophotometric Determination of Glimepiride in Pharmaceutical
Dosage Forms. International Journal of Pharmaceutical Sciences Review and Research 2013;21(2):131-133.
11. Bonfilio R, de Araújo MB, Salgado HRN. Development and Validation of UV-Derivative Spectrophotometric Method for Determination
of Glimepiride in Tablets. J. Braz. Chem. Soc. 2011;22(2):292-299.
12. Chafle D, Awale L. Development and validation of direct spectrophotometric method for estimation of glimepiride. J. Pharm. Res. Int.
2021;33(30A):176–186.
13. Arayne MS, Sultana N, Zuberi MH. Development and validation of RP-HPLC method for analysis of metformin. Pakistan Journal of
Pharmaceutical Sciences 2006;19(3):231-235.
14. Tache F, Albu M. Specificity of analytical HPLC method for metformin hydrochloride . Revue Roumaine de Chimie. 2007;52(6):603-
609.
15. Mubeen G, Noor K. Spectrophotometric determination of metformin hydrochloride. Indian J Pharm Sci, 2009;71(3):318-320.
16. Patel MM, Patel Divya, Shah U, Kachhiya HM Development and validation of a simple, precise and sensitive RP-HPLC method for the
quantification of Teneligliptin Hydrobromide and Metformin Hydrochloride. Research Journal Of Pharmacy And Technology
2023;16(2):495-501.
17. Kumar D, Ramakrishna R, Srivastava S, Dixit RK. RP-HPLC-PDA method development and validation for determination of metformin
in bulk and dosage form. World Journal of Pharmacy and Pharmaceutics Sciences. 2014;3(8):745-757.
18. Joshi SS, Nahire RR, Shastri NR, Surendranath KV, Satish J Validated stability-indicating RP-HPLC UV method for simultaneous
determination of metformin and repaglinide. Acta Chromatographica. 2012;24(2):319-332.
19. Raza A, Murtaza SH, Hanif S, Iqbal J, Ali I, Aftab T et al. Validation of a rapid and economical RP-HPLC method for simultaneous
determination of metformin hydrochloride and sitagliptin phosphate monohydrate: greenness evaluation using AGREE score. Pak. J.
Pharm. Sci. 2023;36(3):. 2022;35(1):15-21. 20. Nirupa G, Tripathi UM. RP-HPLC Analytical Method Development and Validation for
Simultaneous Estimation of Three Drugs: Glimepiride, Pioglitazone and Metformin and Its Pharmaceutical Dosage Forms. Journal of
Chemistry . 2013;2013:Article ID 726235.
20. Chhetri HP, Thapa P, Van Schepdael A. Determination of metformin hydrochloride in bulk and dosage forms by HPLC. International
Journal of Pharmaceutical Sciences and Research. 2013;4(7):2600-2604.
21. Karthik A, Subramanian G, Mallikarjuna Rao C, et al. RP-HPLC method for simultaneous determination of pioglitazone and glimepiride
in bulk drug and pharmaceutical dosage form. Pak J Pharm Sci. 2008;21(4):421-5.
22. Zhou S, Zuo P, Zuo Y, Deng Y. Rapid determination of glimepiride in pharmaceutical formulations by hydrophilic interaction liquid
chromatography. Journal of Saudi Pharmaceutical Society 2017;25(5):760-766.
23. Pawar S, Meshram G, Jadhav R, Bansal Y. Simultaneous estimation of impurities of Glimepiride and Metformin hydrochloride in
sustained release pharmaceutical drug product by HPLC. Der Pharma Chemica. 2010; 2(4): 157-168.
24. Kumar A, Sharma AK, Dutt R. Development and validation of reverse phase high performance liquid chromatography method for
estimation of glimepiride in tablet and bulk form. International Journal of Pharma Quality Assurance 2020;11(2):296-302.
25. Bonfilio R, de Araújo MB, Salgado HRN. Development and Validation of a UV-Derivative Spectrophotometric Method for Determination
of Glimepiride in Tablets. Journal of the Brazilian Chemical Society 2011;22(2):292-299.
26. Useni Reddy M, Viswanath Reddy P, Somasekhar P, Bobbarala V. RP-HPLC method development and validation for the determination
of six anti diabetic drug products. J. Pharm. Res. 2011;4(4):1209-1212. 28. Navghare AB, Shirsat MK. Development, Validation, and
Stability RP-HPLC method for estimation of Metformin and Sitagliptin in Pharmaceutical dosage form. International Journal of Advance
Engineering Science and Management. 2022;18(1):148-51.
27. 29. Vaingankar PN, Amin PD. Development and Validation of Stability Indicating RP-HPLC Method for Simultaneous Estimation of
Metformin HCI and Glimepiride in Fixed Dose Combination. Analytical Chemistry Views. 2016;11:13-20.
28. 30. Vaingankar PN, Amin PD. Development and Validation of Stability Indicating RP-HPLC Method for Simultaneous Determination of
Metformin HCI and Glimepiride in Fixed Dose Combination. Analytical Chemistry View 2016;11:13-20.
29. 31. Rao AS, Nalanda RB, Natraj SK. Development and validation of a stability indicating RP-HPLC method for metformin hydrochloride
and glimepiride in bulk and pharmaceutical dosage form. World Journal of Pharmaceutical Sciences. 32. Srinivas A, Sathish R, Agaiah
Goud B. Method Development, Validation for Simultaneous Estimation of Metformin Hydrochloride and Glimepiride in Bulk and Tablet
Dosage Form by Using RP-HPLC and Its Degradation Studies. 2016; 4(9): 433-441. Journal of Advanced Pharmaceutical Science.
2014;4(1):532-538.
30. 33. Rachh PR, Rahane RD. Development and Validation of Simultaneous Estimation of Metformin Hydrochloride and Glimepiride in
Physiological Media (“Journal name not provided in excerpt”)
31. 34. Badyal PN, Sharma C, Shankar R, Rawal RK. First Generation Chemometric Methods for Simultaneous Determination of Metformin
Hydrochloride, Glimepiride and Pioglitazone. Asian Journal of Biomedical and Pharmaceutical Sciences. 2015;5(47):23-29.
32. 35. Srivastav A, Tiwari P, Maheshwari S. Development and Validation of Simultaneous Equation UV-Spectrophotometric Method for
the Estimation of Metformin HCl and Glimepiride in Combined Dosage Form. (Journal name not given in excerpt)..

Related Articles

2026

A Strategic Framework for Depth-Dependent Hydroelectric Conversion along the Indian Coastline

2026

Reimagining Development in India: A Critical Analysis of the Viksit Bharat Vision

2026

AI-Enabled Image Description: Bridging the Gap for the Visually Impaired

2026

Perceived Occupational Risks of Emergency Medical Services Personnel

2026

Origin, Growth and recent Development of Integrated Reporting (IR): A theoretical Review

2026

Smart Hostel Management System

Share Article

X
LinkedIn
Facebook
WhatsApp

Or copy link

https://www.ijrtmr.com/archives/a-critical-review-of-uv-spectrophotometry-and-hplc-methods-for-the-quantitative-determination-of-metformin-hydrochloride-and-glimepiride

*Instagram doesn't support direct link sharing from web. Copy the link and share it in your Instagram story or post.