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Background: The rapid adoption of Artificial Intelligence (AI) and intelligent automation is reshaping organizational structures, job roles, and workforce requirements. Workforce 5.0 emphasizes a human-centric approach where employees and AI systems collaborate to enhance productivity, innovation, and organizational adaptability. Objective: This study aims to explore Human–AI collaboration models that facilitate workforce reskilling, talent development, and organizational agility in intelligent enterprises. It seeks to identify strategies that enable organizations to prepare employees for emerging digital work environments. Methodology: A qualitative and conceptual research approach was employed through an extensive review of recent literature on AI adoption, workforce transformation, reskilling frameworks, and organizational agility. Relevant academic publications, industry reports, and digital transformation studies were analyzed to develop a Workforce 5.0 collaboration framework. Results: The findings indicate that effective Human–AI collaboration significantly improves operational efficiency, decision-making quality, employee engagement, and innovation capability. AI-supported learning platforms, adaptive reskilling programs, and intelligent talent management systems enable organizations to respond rapidly to changing business demands while enhancing workforce readiness. Conclusion: Workforce 5.0 presents a strategic pathway for organizations seeking sustainable growth in the era of intelligent enterprises. By integrating AI technologies with human creativity, critical thinking, and emotional intelligence, organizations can build agile, resilient, and future-ready workforces. The proposed framework provides practical guidance for implementing human-centered digital transformation and continuous workforce development.