ARCHIVES
Year 2026 · Volume 6 · Issue 4
Original Article
An Eco-Friendly UV Spectrophotometric Method for the Determination of 5,7-Dihydroxyflavone in Bulk Drug
Arif Salim Jamadar1
Ronak Dsouza2
Adarsh Anil Kamate3
Samarth Ugare4
Shailendra Sanjay Suryawanshi5
1 2 3 5Department of Pharmaceutical Analysis, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Belagavi, Karnataka, India. 4 Department of Pharmaceutical Chemistry, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Belagavi, Karnataka, India. *Corresponding Author.
Published Online: July-August 2026
Pages: 396-403
Cite this article
↗ https://www.doi.org/10.59256/ijrtmr.20260604043References
1. Li S, Han Q, Qiao C, Song J, Lung Cheng C, Xu H. Chemical markers for the quality control of herbal medicines: an overview. Chin
Med. 2008;3:7.
2. Kasala ER, Bodduluru LN, Madana RM, V AK, Gogoi R, Barua CC. Chemopreventive and therapeutic potential of 5,7-Dihydroxyflavone
in cancer: mechanistic perspectives. Toxicol Lett. 2015;233(2):214-225.
3. Skoog DA, Holler FJ, Crouch SR. Principles of Instrumental Analysis. 7th ed. Boston: Cengage Learning; 2018.
4. International Council for Harmonisation (ICH). ICH Harmonised Guideline Q2(R2): Validation of Analytical Procedures. Geneva: ICH;
2023.
5. Suryawanshi SS, Zaranappa, Chaluvaraju KC, Veena MK, Rajani S. Development and validation of UV-spectrophotometric method for
simultaneous estimation of aceclofenac and pantoprazole in bulk and tablet dosage forms using hydrotropic solvent. Int J Pharm Pharm
Res. 2016;6(3)
6. Pancham YP, Girish B, Suryawanshi SS. UV-spectrophotometric method for quantification of ascorbic acid in bulk powder. Pharma
Innov J. 2020;9(5):5-8.7. Suryawanshi SS, Kavalapure R, Palled MS, Alegaon SG. Development and validation of UV-spectrophotometric method for
determination of ciprofloxacin and curcumin in bulk powder. Int J Pharm Sci Res. 2020;11(3):1161-1166. doi:10.13040/IJPSR.0975-
8232.11(3).1161-66.
8. Tirodkar CU, Palled MS, Suryawanshi SS, Majukar SM. Development and validation of stability indicating UV-spectrophotometric
method for the simultaneous estimation of telmisartan and metformin hydrochloride in bulk drugs. Indian J Pharm Educ Res.
2021;55(2):590-596
9. Kagawad P, Gharge S, Jivaje K, Hiremath SI, Suryawanshi SS. Quality control and standardization of quercetin in herbal medicines by
spectroscopic and chromatographic techniques. Future J Pharm Sci. 2021;7:176. doi:10.1186/s43094-021-00327-y.
10. Hiremath SI, Palled MS, Suryawanshi SS, Chouhan MK. Development and standardization of UV-spectrophotometric method for
estimation of azadirachtin in marketed formulation. Int J Pharm Res. 2021;13(3). doi:10.31838/ijpr/2021.13.03.063.
11. Yadav RK, Maste MM, Surywanshi SS, Shastri U. Conductometric method development and validation to estimate acamprosate calcium
in API and marketed formulation. J Appl Pharm Sci. 2021;11(11):82-86. doi:10.7324/JAPS.2021.1101111.
12. Supe OS, Maste MM, Suryawanshi SS, Yadav RK. Development and standardization of stability indicating UV-spectrophotometric
method for assessment of bilastine in bulk and pharmaceutical dosage formulation. Int J Pharm Sci Res. 2022;13(2):962-968.
doi:10.13040/IJPSR.0975-8232.13(2).962-68.
13. Patil A, Maste MM, Suryawanshi SS, Patil N. Green solvent assisted UV-spectrophotometric method for estimation of rosuvastatin in
bulk and pharmaceutical dosage forms. Res J Pharm Technol. 2022;15(2):587-590. doi:10.52711/0974-360X.2022.00096.
14. Salokhe AS, Malkar S, Mangule S, Palled MS, Suryawanshi SS, Dandagi PM. Validated area under curve and zero order spectroscopic
methods for estimation of agomelatine in bulk and pharmaceutical tablet dosage form. Biomed Pharmacol J. 2022;15(3):1337-1348.
doi:10.13005/bpj/2470.
15. Tavade S, Patil K, Kurangi B, Suryawanshi SS. Development and validation of UV-spectrophotometric method for estimation of
berberine hydrochloride in marketed formulation and poly lactic co-glycolic acid nanoparticles. Indian J Pharm Educ Res.
2022;56(3):873-880. doi:10.5530/ijper.56.3.140.
16. Chougule SB, Jivaje AB, Mannur VS, Suryawanshi SS. Validated spectroscopic and chromatographic techniques for quality control
analysis of apigenin in herbal medicines. Int J Pharm Sci Res. 2022;13(12):4857-4864. doi:10.13040/IJPSR.0975-8232.13(12).4857-64.
17. Surve RR, Tavanoji HP, Nadaf TR, Pote SC, Maste MM, Suryawanshi SS. Marker based standardization of quercetin in marketed
capsules by novel zero order spectroscopic and area under curve spectroscopic methods. Indian J Pharm Educ Res. 2023;57(3
Suppl):S772-S786. doi:10.5530/ijper.57.3s.82.
18. Suryawanshi SS, Sampat G, Palled MS, Patil AS. A green chemistry approach to establish a conductometric technique for quantifying
metformin HCl in pharmaceutical samples and its greenness assessment using an analytical greenness metric calculator. J Appl Pharm
Sci. 2023;13(10):238-244. doi:10.7324/JAPS.2023.145086.
19. Bhusannavar D, Sharannavar B, Darbha NS, Suryawanshi SS, Patil S, Bhandurge P. Development and validation of UV-visible
spectrophotometry method for simultaneous estimation of rutin and curcumin in bulk pharmaceuticals. Int J Pharm Investig.
2024;14(3):833-842. doi:10.5530/ijpi.14.3.93
20. Sharannavar B, Amonkar MB, Deo A, Suryawanshi SS, Darbha NS. Simultaneous estimation of minoxidil and tofacitinib citrate in bulk
and topical gel: A validated UV spectrophotometric technique. Adv Pharm Pharm. 2025;13(1):37-49. doi:10.13189/app.2025.130105.
21. Patil AA, Suryawanshi SS. Computer aided drug design approach for repurposing of vitamins and related compounds in drug discovery
through drug-likeness and bioavailability analysis. KLE J Pharm Health Sci Res. 2025;1(1):57-63.
22. Jamadar AS, Suryawanshi SS. Advanced analytical approaches for salbutamol sulphate: A comprehensive review of techniques,
challenges, and future trends. Int J Pharm Pharm Sci. 2025;7(2):1-4. doi:10.33545/26647222.2025.v7.i2a.186.
23. Pakali SS, Palled MS, Suryawanshi SS. Quality by design-assisted green UV-spectrophotometric method development and validation
for the quantitative estimation of letrozole. Drug Anal Res. 2025;9(1):32-41. doi:10.22456/2527-2616.147251.
24. Bandekar SS, Suryawanshi SS, Palled MS, Dinnimath B. Alpinia galanga (L.) Willd: A comprehensive review on phytochemistry,
pharmacology, analytical characterization, nanotechnology applications, clinical evidences and safety profile. World J Biol Pharm Health
Sci. 2025;24(1):193-201. doi:10.30574/wjbphs.2025.24.1.0760.
25. Patil RN, Suryawanshi SS, Palled MS. Bioanalysis of chrysin in human plasma and analytical stability using validated UV-
spectrophotometric method: A quality by design approach. Drug Anal Res. 2025;9(2):43-52. doi:10.22456/2527-2616.148193.
26. Palled MS, Suryawanshi SS, Revankar SJ, Pandare SM. Design of experiment based UV-spectrophotometric method for quality control
analysis of silymarin in marketed tablet formulation. Indian Drugs. 2025;62(4):37-47. doi:10.53879/id.62.04.14757.
27. Bandekar SS, Suryawanshi SS, Patil VV, Bhogan ST, Patil P, Palled MS, Pujari RA. A method for eco-sustainable quantification of 4-
hydroxy-3-methoxy-cinnamic acid in the rhizome extract of Alpinia galanga (L.) Willd., using analytical quality by design approach. J
Chem Health Risks. 2026;16(2):2796-2808.
28. Bhatkande AV, Palled MS, Bhingurde TA, Gawas S, Bhatkande AA, Dsouza R, Biradar S, Patil VV, Suryawanshi SS, Jamadar AS. Eco-
Sustainable UV Spectrophotometric Quantification of Dapagliflozin in Marketed Formulations Using Area Under Curve Approach:
Validation and Greenness Assessment. Indian Journal of Science and Technology. 2026 Jun 16;19(21):1449-57.
29. Gavali KJ, Maste MM, Gawas NS, Suryawanshi S, Shetti PP. A quality by design (QbD) approach for the development and validation
of an RP-HPLC method for azelnidipine quantification. Adv Pharm Pharm. 2026;14(4):460-79. doi:10.13189/app.2026.140404.
30. Revankar SJ, Pandare SM, Palled MS, Suryawanshi SS. A Quality by Design (QBD) approach for the development and validation of
RP-HPLC method for the quantification of silymarin tablet. Int J Adv Pharm Sci Res. 2024;4(5):9-15.
doi:10.54105/IJAPSR.E4046.04050824.
Med. 2008;3:7.
2. Kasala ER, Bodduluru LN, Madana RM, V AK, Gogoi R, Barua CC. Chemopreventive and therapeutic potential of 5,7-Dihydroxyflavone
in cancer: mechanistic perspectives. Toxicol Lett. 2015;233(2):214-225.
3. Skoog DA, Holler FJ, Crouch SR. Principles of Instrumental Analysis. 7th ed. Boston: Cengage Learning; 2018.
4. International Council for Harmonisation (ICH). ICH Harmonised Guideline Q2(R2): Validation of Analytical Procedures. Geneva: ICH;
2023.
5. Suryawanshi SS, Zaranappa, Chaluvaraju KC, Veena MK, Rajani S. Development and validation of UV-spectrophotometric method for
simultaneous estimation of aceclofenac and pantoprazole in bulk and tablet dosage forms using hydrotropic solvent. Int J Pharm Pharm
Res. 2016;6(3)
6. Pancham YP, Girish B, Suryawanshi SS. UV-spectrophotometric method for quantification of ascorbic acid in bulk powder. Pharma
Innov J. 2020;9(5):5-8.7. Suryawanshi SS, Kavalapure R, Palled MS, Alegaon SG. Development and validation of UV-spectrophotometric method for
determination of ciprofloxacin and curcumin in bulk powder. Int J Pharm Sci Res. 2020;11(3):1161-1166. doi:10.13040/IJPSR.0975-
8232.11(3).1161-66.
8. Tirodkar CU, Palled MS, Suryawanshi SS, Majukar SM. Development and validation of stability indicating UV-spectrophotometric
method for the simultaneous estimation of telmisartan and metformin hydrochloride in bulk drugs. Indian J Pharm Educ Res.
2021;55(2):590-596
9. Kagawad P, Gharge S, Jivaje K, Hiremath SI, Suryawanshi SS. Quality control and standardization of quercetin in herbal medicines by
spectroscopic and chromatographic techniques. Future J Pharm Sci. 2021;7:176. doi:10.1186/s43094-021-00327-y.
10. Hiremath SI, Palled MS, Suryawanshi SS, Chouhan MK. Development and standardization of UV-spectrophotometric method for
estimation of azadirachtin in marketed formulation. Int J Pharm Res. 2021;13(3). doi:10.31838/ijpr/2021.13.03.063.
11. Yadav RK, Maste MM, Surywanshi SS, Shastri U. Conductometric method development and validation to estimate acamprosate calcium
in API and marketed formulation. J Appl Pharm Sci. 2021;11(11):82-86. doi:10.7324/JAPS.2021.1101111.
12. Supe OS, Maste MM, Suryawanshi SS, Yadav RK. Development and standardization of stability indicating UV-spectrophotometric
method for assessment of bilastine in bulk and pharmaceutical dosage formulation. Int J Pharm Sci Res. 2022;13(2):962-968.
doi:10.13040/IJPSR.0975-8232.13(2).962-68.
13. Patil A, Maste MM, Suryawanshi SS, Patil N. Green solvent assisted UV-spectrophotometric method for estimation of rosuvastatin in
bulk and pharmaceutical dosage forms. Res J Pharm Technol. 2022;15(2):587-590. doi:10.52711/0974-360X.2022.00096.
14. Salokhe AS, Malkar S, Mangule S, Palled MS, Suryawanshi SS, Dandagi PM. Validated area under curve and zero order spectroscopic
methods for estimation of agomelatine in bulk and pharmaceutical tablet dosage form. Biomed Pharmacol J. 2022;15(3):1337-1348.
doi:10.13005/bpj/2470.
15. Tavade S, Patil K, Kurangi B, Suryawanshi SS. Development and validation of UV-spectrophotometric method for estimation of
berberine hydrochloride in marketed formulation and poly lactic co-glycolic acid nanoparticles. Indian J Pharm Educ Res.
2022;56(3):873-880. doi:10.5530/ijper.56.3.140.
16. Chougule SB, Jivaje AB, Mannur VS, Suryawanshi SS. Validated spectroscopic and chromatographic techniques for quality control
analysis of apigenin in herbal medicines. Int J Pharm Sci Res. 2022;13(12):4857-4864. doi:10.13040/IJPSR.0975-8232.13(12).4857-64.
17. Surve RR, Tavanoji HP, Nadaf TR, Pote SC, Maste MM, Suryawanshi SS. Marker based standardization of quercetin in marketed
capsules by novel zero order spectroscopic and area under curve spectroscopic methods. Indian J Pharm Educ Res. 2023;57(3
Suppl):S772-S786. doi:10.5530/ijper.57.3s.82.
18. Suryawanshi SS, Sampat G, Palled MS, Patil AS. A green chemistry approach to establish a conductometric technique for quantifying
metformin HCl in pharmaceutical samples and its greenness assessment using an analytical greenness metric calculator. J Appl Pharm
Sci. 2023;13(10):238-244. doi:10.7324/JAPS.2023.145086.
19. Bhusannavar D, Sharannavar B, Darbha NS, Suryawanshi SS, Patil S, Bhandurge P. Development and validation of UV-visible
spectrophotometry method for simultaneous estimation of rutin and curcumin in bulk pharmaceuticals. Int J Pharm Investig.
2024;14(3):833-842. doi:10.5530/ijpi.14.3.93
20. Sharannavar B, Amonkar MB, Deo A, Suryawanshi SS, Darbha NS. Simultaneous estimation of minoxidil and tofacitinib citrate in bulk
and topical gel: A validated UV spectrophotometric technique. Adv Pharm Pharm. 2025;13(1):37-49. doi:10.13189/app.2025.130105.
21. Patil AA, Suryawanshi SS. Computer aided drug design approach for repurposing of vitamins and related compounds in drug discovery
through drug-likeness and bioavailability analysis. KLE J Pharm Health Sci Res. 2025;1(1):57-63.
22. Jamadar AS, Suryawanshi SS. Advanced analytical approaches for salbutamol sulphate: A comprehensive review of techniques,
challenges, and future trends. Int J Pharm Pharm Sci. 2025;7(2):1-4. doi:10.33545/26647222.2025.v7.i2a.186.
23. Pakali SS, Palled MS, Suryawanshi SS. Quality by design-assisted green UV-spectrophotometric method development and validation
for the quantitative estimation of letrozole. Drug Anal Res. 2025;9(1):32-41. doi:10.22456/2527-2616.147251.
24. Bandekar SS, Suryawanshi SS, Palled MS, Dinnimath B. Alpinia galanga (L.) Willd: A comprehensive review on phytochemistry,
pharmacology, analytical characterization, nanotechnology applications, clinical evidences and safety profile. World J Biol Pharm Health
Sci. 2025;24(1):193-201. doi:10.30574/wjbphs.2025.24.1.0760.
25. Patil RN, Suryawanshi SS, Palled MS. Bioanalysis of chrysin in human plasma and analytical stability using validated UV-
spectrophotometric method: A quality by design approach. Drug Anal Res. 2025;9(2):43-52. doi:10.22456/2527-2616.148193.
26. Palled MS, Suryawanshi SS, Revankar SJ, Pandare SM. Design of experiment based UV-spectrophotometric method for quality control
analysis of silymarin in marketed tablet formulation. Indian Drugs. 2025;62(4):37-47. doi:10.53879/id.62.04.14757.
27. Bandekar SS, Suryawanshi SS, Patil VV, Bhogan ST, Patil P, Palled MS, Pujari RA. A method for eco-sustainable quantification of 4-
hydroxy-3-methoxy-cinnamic acid in the rhizome extract of Alpinia galanga (L.) Willd., using analytical quality by design approach. J
Chem Health Risks. 2026;16(2):2796-2808.
28. Bhatkande AV, Palled MS, Bhingurde TA, Gawas S, Bhatkande AA, Dsouza R, Biradar S, Patil VV, Suryawanshi SS, Jamadar AS. Eco-
Sustainable UV Spectrophotometric Quantification of Dapagliflozin in Marketed Formulations Using Area Under Curve Approach:
Validation and Greenness Assessment. Indian Journal of Science and Technology. 2026 Jun 16;19(21):1449-57.
29. Gavali KJ, Maste MM, Gawas NS, Suryawanshi S, Shetti PP. A quality by design (QbD) approach for the development and validation
of an RP-HPLC method for azelnidipine quantification. Adv Pharm Pharm. 2026;14(4):460-79. doi:10.13189/app.2026.140404.
30. Revankar SJ, Pandare SM, Palled MS, Suryawanshi SS. A Quality by Design (QBD) approach for the development and validation of
RP-HPLC method for the quantification of silymarin tablet. Int J Adv Pharm Sci Res. 2024;4(5):9-15.
doi:10.54105/IJAPSR.E4046.04050824.
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