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Background: The study aims to explore environmental sustainability and medical waste management practices in Ghanaian private hospitals in the Greater Accra Region. It also sought to determine if there is any form of collaboration between private hospitals and district assemblies in managing medical wastes. Methods: A qualitative research approach with a cross-sectional study design was used to collect data from employees of the three private hospitals and the district assemblies. A structured interview guide was employed to gather data from 13 purposively selected participants. Results: The findings of the study indicated that there is a collaboration between private hospitals and district assemblies with regard to medical wastes management activities. Conclusions: It is recommended that policies on medical waste management are enforced to ensure sustainable healthcare waste management.